BARX2/FOXA1/HK2 axis promotes lung adenocarcinoma progression and energy metabolism reprogramming
Introduction
Lung cancer is the most common fatal cancer worldwide, with over 2.2 million new cases and 1.78 million deaths annually (1). Non-small-cell cancer (NSCLC), including lung adenocarcinoma (LUAD) and lung squamous cell carcinomas (LUSC), accounts for 80–85% of all cases (2). Despite advances in cancer treatment, LUAD patients’ prognosis is far from satisfying (3). Although treatment of LUAD has met with significant progress in recent times, the overall survival (OS) of LUAD patients remains suboptimal, and valuable molecular biomarkers are lacking. Consequently, investigating the underlying pathogenesis of LUAD is of significant importance in improving its early diagnosis and effective treatment.
Cancer cells are often subjected to various environmental stresses such as nutritional deficiencies, hypoxia, and acidosis (4), and to fulfil energy and biosynthetic needs, they metabolize substrates such as glucose and fatty acids through metabolic reprogramming (5). The aberrant metabolic characteristic of tumors is considered a hallmark of cancer, and cancer cells preferentially utilize the glycolytic pathway to produce more pyruvate and lactate even when sufficient oxygen is available, leading to acidification of the microenvironment (5,6). This phenomenon is also termed Warburg effect, in which cancer cells grow through differential uptake of glucose and amino acids, and increased biosynthesis of lipids, proteins, and nucleic acid (7). Warburg effect within the tumor microenvironment results in an accumulation of lactic acid, which acidifies the tumor microenvironment. In recent studies, it has been shown that tumor cells can escape immune surveillance due to an accumulation of lactic acid (8,9). An increasing number of studies have aimed to explore the actual link between metabolic processes and oncogenesis (10), and pharmacological agents targeting critical regulators of glucose metabolism are in development (11,12). It is well known that transcription factors are critical in regulating the Warburg effect (13), including p53, MYC, and HIF-1α which are responsible for the metabolic switch from oxidative phosphorylation to the glycolysis pathway in tumor cells.
Homeodomain transcription factors participate in tumorigenesis and developmental processes by controlling cellular behaviour such as proliferation, adhesion, migration, differentiation, and apoptosis (14,15). The BarH-like homeobox 2 (BARX2) gene is a homeodomain factor in the Bar family located in this critical LOH region 11q24-q25 region (16,17), and is a crucial regulator of cell adhesion, cytoskeleton remodeling, and growth factor signaling (18-21). BARX2-deficient mouse embryos show defective heteromorphic regeneration (22). Furthermore, BARX2 plays a pivotal role in many cancers, particularly urinary bladder cancer (23) and gastric cancer (24). Although BARX2 is a transcription factor, its role in regulating tumor metabolism remains unclear. HK2 is the rate-limiting enzyme catalyzes the first irreversible step of glycolysis (25). The increasing evidence indicates that HK2 overexpression and poor prognosis are strongly associated in multiple human cancers (26-28). Forkhead box A1 (FOXA1) is a member of a group of transcription factors known as pioneer factors. FOXA1 binds to condensed chromatin and allows subsequent binding of other transcription factors (29). Several studies have shown that FOXA1 plays roles oncogenic in NSCLC cells (30,31).
In this study, we aimed to determine metabolism-related transcription factors and discover their underlying mechanisms. Our analyses demonstrated that BARX2 is frequently upregulated in LUAD, and its upregulation is linked to poorer patient survival. In vitro and in vivo functional experiments indicated BARX2 may regulate cell proliferation and tumorigenicity. Mechanistically, our findings suggest that the BARX2/FOXA1/HK2 axis promotes LUAD progression and energy metabolism reprogramming. We present the following article in accordance with the ARRIVE reporting checklist (available at https://tlcr.amegroups.com/article/view/10.21037/tlcr-22-465/rc)
Methods
Clinical specimens
LUAD and corresponding adjacent normal lung tissues were harvested from 2017–2021 at the Department of Thoracic Surgery, Jiangsu Cancer Hospital. The samples were snap-frozen in liquid nitrogen immediately after resection. The study was conducted in accordance with the Declaration of Helsinki (as revised in 2013). The study was approved by ethics board of Jiangsu Cancer Hospital. Informed consent was taken from all the patients.
Cell culture
All cell lines [PC9, A549, NCI-1975, NCI-H1299, and human bronchial epithelial cell (HBE)] were purchased from the Shanghai Institute for Biological Science, China. PC9, A549, NCI-1975, and NCI-H1299 cells were cultivated in DMEM (KeyGene, Nanjing, China) with high glucose (4.5 g/L) and 10% fetal bovine serum, and HBE cells were cultivated in RPMI 1640 (KeyGene, Nanjing, China), supplemented with 10% fetal bovine serum. All cell lines were maintained in a humidified incubator at 37 °C (5% CO2).
Tissue microarrays (TMAs) and immunohistochemistry (IHC)
LUAD TMAs, obtained from Shanghai Outdo Biotech Co. Ltd. (Cat. No. HLugA180Su04) was analyzed for BARX2 expression. The pathologic stage was determined according to the Union for International Cancer Control (UICC) TNM staging system (8th edition). The sections were incubated with an anti-BARX2 antibody (Clone ARG-10900; arigo Biolaboratories, Shanghai, China; 1:1,000) and the IHC scores were calculated according to intensity and percentage of positive cells. The staining intensity was scored as following: 0 (negative), 1 (weak), 2 (moderate), or 3 (strong). The H score (percentage of positive cells multiplied by respective intensity scores) was used as the final staining score (a minimum value of 0 and a maximum value of 300).
IHC assays were performed as standard protocols. In brief, mouse tissue sections were deparaffinized in xylene and rehydrated in graded alcohol (100%, 96%, 70%). Endogenous peroxidase activity was blocked by 3% hydrogen peroxide incubation and antigen extraction, while the antibody used for immunostaining was the anti-BARX2 antibody.
RNA extraction and quantitative RT-PCR
Total RNA was isolated using Trizol reagent (Invitrogen) from cells or tissue samples according to the manufacturer’s guidelines. Relative RNA level was measured using the SYBR Select Master Mix (Applied Biosystems, Cat: 4472908. KeyGEN, Nanjing, China) by quantitative real-time polymerase chain reaction (qRT-PCR), using GAPDH as endogenous control. The relative amount levels of RNA were calculated using the comparative 2−ΔΔCT method. All primer sequences are listed in Table S1.
Western blotting
Western blot assays were performed according to the manufacturer’s protocol (32). Anti-β-actin antibody (Clone 3700; Cell Signaling Technology, Danvers, Massachusetts, USA; 1:1,000) was used as a loading control. The antibodies were purchased from the following manufacturers: anti-BARX2 (Clone ARG-10900; arigo Biolaboratories, Shanghai, China; 1:1,000), anti-FOXA1 (Clone 53528S; Cell Signaling Technology, Danvers, Massachusetts, USA; 1:1,000), anti-HK2 (Clone 2867T; Cell Signaling Technology, Danvers, Massachusetts, USA; 1:1,000).
Plasmid vectors and siRNAs transfection
Plasmid vectors or siRNAs were transfected with Lipofectamine™ 3000 Transfection Reagent (Invitrogen, CA, USA) or Lipofectamine RNAiMAX (Invitrogen, CA, USA), respectively, according to the manufacturer’s guidelines. The specific siRNA and transfection plasmid vectors were purchased from RiboBio. All siRNA sequences are shown in Table S2.
Cell proliferation and migration/invasion assays
After 24 hours (h) of transfection, cells were harvested for assessment of cell proliferation, which was then examined by a cell counting kit-8 (CCK-8). Cells were plated overnight in 96-well plates at a controlled density of 2,000 cells per well, then CCK-8 (10 µL/well) was added to each well before incubation for 2 h. The reaction products were measured at 450 nm according to the manufacturer’s instructions. xCELLigence analysis system (RTCA) was performed to monitor real-time cell proliferation, and 8,000 cells per well were added on E-plates 16, with the proliferation curve tested every half hour for cell index up to 90 h. For the EdU incorporation experiment, the Cell-Light™ EdU Apollo®488 In Vitro Imaging Kit (RiboBio, Guangzhou, China) was used and the assay was performed according to the instructions. To calculate the number of cells: Open ImageJ software, choose File->Open (select the image to Analyze), and Plugins->Analyze->CellCounter. First click Initialize, and then select the cell types to be counted respectively. Define the blue one as Type 1 and the green one as Type 2. Select Type 1 and click the blue dot in the picture one by one, and the total number of cells will be displayed in the box after Type 1. For migration/invasion assays, 5×104 cells were seeded on Transwell kits (8 mm PET, 24-well Millicell) or Matrigel-coated inserts (BD Biosciences, Bedford, MA, USA). After 24 or 48 h of incubation, cells were fixed with methanol and stained with 0.1% crystal violet, imaged, then counted under the microscope. To calculate the number of cells: Open ImageJ software, go to Type ->8-bit to change the Image from RGB to 8-bit, then go to Edit -> Invert, then Image -> Adjust -> Threshold... To open the threshold window and set the threshold. Adjust the Threshold through the Threshold window Threshold, and the cells whose brightness exceeds the Threshold will be counted. After setting, click the Apply button to Apply it to the picture. If two, three or more cells are found to overlap, you can separate the cells using the Watershed method by going to Process -> Binary -> Watershed. Then click Analyze -> Analyze Particles..., set the nucleus size, select display outline or other options. Click OK to analyze these points of interest. Three Windows will pop up, namely Summary, Results and ROI Manager, summarizing the total number of these points (nucleus) and various parameters of each point (such as area, average value, width, height, etc.). Each point can be manipulated (add or delete, etc.) through ROI Manager. Finally, you can see the total number of cells in the image in the Summary Windows. Each experiment was determined in triplicate, independently.
Metabolic assays
A Glucose Uptake Assay Kit (Abcam, Cambridge, UK), L-lactate Assay Kit (Abcam, Cambridge, UK), Pyruvate Assay Kit (Abcam, Cambridge, UK), and Seahorse XF Glycolysis Stress Test kit (Agilent, Beijing, China) were used for detecting glucose, lactate, pyruvate, and ECAR, respectively, and performed according to the standard protocols. Cells were seeded in 6-well plates and cultured overnight, before the supernatant was collected, and glucose, lactate, and pyruvate were measured. For ECAR detection, cells were seeded in XF 24-well plates with 1×104 cells and starved overnight. ECAR was monitored by the Seahorse XF 24 Extracellular Flux Analyzer (Agilent, Beijing, China). The results were normalized to the protein content, and each experiment was determined in triplicate independently.
Chromatin immunoprecipitation (ChIP)
ChIP assays were performed by ChIP Assay Kit (Sigma-Aldrich) according to the manufacturer’s protocol (32). We used 5 µg anti-BARX2 antibody or 5 µg anti-FOXA1 antibody for each reaction, and 5 µg mouse IgG (Abcam; Cat: ab-150115) was applied as the control. The purified ChIP DNA samples were subjected to PCR amplification. All specific primer sequences are listed in Tables S3. GAPDH was used as control.
Luciferase reporter assay
Luciferase reporter assays were assessed by the Dual-Luciferase Reporter Assay System (Promega, Madison, WI, USA). The 3'UTR of FOXA1 or BARX2 containing the predicted binding site for BARX2 or HK2 was cloned into a pGL4-basic luciferase reporter vector. CMV-Renilla was applied as control. The luciferase activity was measured by Firefly/Renilla value. Transfection was performed using Lipofectamine™ 3000 Transfection Reagent (Invitrogen, CA, USA) according to the manufacturer’s recommendations.
Xenograft model
To avoid possible effects of sex on mice survival, tumor growth, and tumor implantation response, mice of the same sex were used. In comparison to male mice, female mice are more docile and easier to work with. Twelve female nude BALB/c mice purchased from Nanjing Medical University at 4–6 weeks and in good growth condition were randomly divided into two groups. PC9 cells were cultured in large dishes until logarithmic growth phase and transfected when the density reached 70–80% with BARX2 interference plasmid (sh-BARX2) and control plasmid (sh-NC) respectively. Cells were then counted 48 h after transfection following trypsin digestion, washed with phosphate buffered saline (PBS), and resuspended in serum-free medium. We then selected 1×107 cells and suspended them in PBS to construct a subcutaneous lotus tumor model. Mice were housed in 485×350×200 mm cages and were fed with a full-price pellet diet under conditions of free water, room temperature 22±2 °C, humidity 50–60%, and a well ventilated 12:12 h light/dark cycle. Tumor longitudinal and transverse diameters were measured weekly, and tumor volume (TV) was calculated by the formula: TV =1/2 × longer diameter × shorter diameter2. The health of the animals was monitored twice a day during the feeding period, and no adverse events were observed. At the start of the experiments animals weighed [mean ± standard deviation (SD)] 17.8±0.3 grams. Nude mice were sacrificed 6 weeks after cell injection, the tumor was excised, weighed, and retained for subsequent experiments. Experimenters were blinded to the pharmacological treatment while processing data and making exclusion decisions. Animal experiments were approved by the ethics board of Nanjing Medical University (No. 2018-122), in compliance with Chinese guidelines for the care and use of animals. The mean value was expressed as mean ± SD, the inter-group analysis was statistically processed by t-test, and the results were statistically analyzed by SPSS (Statistical Package for the Social Science) 22.0.
Statistical analysis
All statistical data analyses were performed with SPSS 22.0 and the data visualization using GraphPad Prism for Windows v.8.0. The results are presented throughout as mean values ± SD from the three experiments independently. The Student t-test identified the continuous variable between the two groups or more than two groups, and P value <0.05 was considered statistically significant difference.
Results
BARX2 is highly expressed in LUAD tissues and correlates with poorer prognosis
To evaluate the role of BARX2 in LUAD, we first compared its mRNA expression between 60 LUAD and corresponding non-tumor tissues by qRT-PCR. These studies revealed that BARX2 expression was remarkably higher in 97% (57/60) of LUAD tissues (Figure 1A). We then assessed whether BARX2 expression was up-regulated in LUAD cells compared with HBE by Western blot and qRT-PCR (Figure 1B,1C). The The Cancer Genome Atlas (TCGA) dataset indicated that BARX2 expression was significantly higher in LUAD samples than in non-neoplastic lung samples (P<0.001) (Figure 1D). Similarly, BARX2 was also markedly higher expressed in 57 LUAD samples than matched non-neoplastic lung samples (P<0.001) (Figure 1E). The receiver operating characteristic (ROC) curves indicated that BARX2 could distinguish LUAD tissue from normal lung tissue with confidence interval (CI) =0.839–0.905 (area under the ROC curve, 0.872) (Figure 1F). Furthermore, the high or low BARX2 expression of LUAD from the TCGA database was defined as a value in the top quartile or bottom quartile of the set, respectively. The overall survival (OS) analysis of patients indicated that high BARX2 expression was associated with poor OS (P=0.043) (Figure 1G).
IHC assays were performed on TMAs to detect the protein expression of BARX2 in 92 human LUAD tissues and 88 adjacent non-neoplastic lung tissues. IHC images of one patient were presented (Figure 1H), and results showed that staining scores of BARX2 were higher in cancer tissues (P<0.001, Figure 1I). Combined with the patients’ clinical information, the expression of BARX2 was higher in patients with more advanced TNM stage (stage I–II vs. III–IV, P<0.001, Figure 1J), T stage (T1-2 vs. T3–T4, P<0.001, Figure 1K), N stage (N0 vs. N1, P<0.001, Figure 1L), and lymph node metastasis (P<0.001, Figure 1M). By ranking the expression of BARX2 in 92 tissues from high to low, we defined the ‘high BARX2 expression’ group (n=46) and the ‘low BARX2 expression’ group (n=46) according to the median level of BARX2 to explore the prognostic significance of BARX2 in LUAD. Survival analysis showed patients with high BARX2 expression had a more dismal OS than patients with low BARX2 expression (P<0.001, Figure 1N).
Together, these data demonstrated BARX2 was highly expressed in LUAD tissues in comparison to adjacent non-neoplastic lung tissue and was associated with poor outcomes.
Knockdown of BARX2 inhibited LUAD cell proliferation and progression in vitro and in vivo
To explore the biological function of BARX2 in LUAD cells, BARX2 was knocked down by transfecting siRNAs-BARX2 into PC9 cells; the successful transfection was verified by qRT-PCR and Western blot (Figure 2A,2B). The results of CCK8 (Figure 2C), RTCA (Figure 2D), and EdU staining assays (Figure 2E,2F) showed that silencing of BARX2 significantly suppressed the proliferation of PC9 cells. In addition, Transwell and Matrigel assays were utilized, measuring the greatly inhibited migration and invasion abilities in siRNAs-BARX2 groups (Figure 2G,2H). Moreover, we transfected plasmids overexpressing BARX2 into A549 cells. The results showed that overexpression promoted the proliferation, migration, and invasion of A549 cells (Figure S1A-S1E).
To investigate the tumorigenic effects of BARX2 in vivo using xenograft tumor models, PC9 cells transfected with NC-shRNA or BARX2-shRNA were injected into nude mice. The results indicated that BARX2-deficient PC9 cells dramatically inhibited tumor growth (volume and weight) (Figure 2I-2K). IHC analysis revealed that tumors derived from sh-BARX2 transfected cells showed less staining of BARX2 than those in the sh-NC group (Figure 2L).
BARX2 contributed to the Warburg effect in LUAD cells
We performed GSEA using the low-BARX2 and high-BARX2 expression datasets to identify the potential function of BARX2 in LUAD, and multiple GSEA showing glycose metabolism-related pathways (Table S4). It is well known that metabolic reprogramming plays a pivotal role in tumorigenesis and is commonly observed in multiple cancers (7,33,34). Metabolic reprogramming is considered an emerging hallmark of cancer (4,35). Tumor cells provide adenosine triphosphate (ATP) for growth mainly through glycolysis and biomolecules for cell replication through the pentose phosphate pathway (36,37). Moreover, even under adequate oxygen conditions, tumor cells preferentially use glycolysis to produce high levels of secreted lactate, which is known as the Warburg effect (Figure 3A). Consequently, we hypothesized that BARX2 might be a key factor for metabolic reprogramming in LUAD cells. As expected, the results showed BARX2 knockdown leads to a reduction in glucose consumption, and lactate and pyruvate production (Figure 3B-3D). Next, we explored whether BARX2 could directly affect the metabolism of LUAD cells by assaying the rate of ECAR (38). Seahorse analysis revealed that ECAR was reduced in PC9 cells by inhibiting the expression of BARX2 (Figure 3E). In addition, overexpression of BARX2 significantly promoted glucose consumption and increased the production of lactate, pyruvate, and ECAR in A549 cells (Figure S2A-S2D). The above results implied that BARX2 contributed to the Warburg effect in LUAD cells.
BARX2 exerts a cancer-promoting effect by regulating HK2
Reprogramming of glucose metabolism in tumors drives the transition to aerobic glycolysis and lactate production by regulating the expression and activation of glycolytic enzymes, including HK2 (hexokinase 2), glucose transporter 1/4 (GLUT1/4, SLC2A1/4), GPI (glucose-6-phosphate isomerase), PFKL (6-phosphofructokinase, liver type), ALDO (aldolase A), GAPDH (glyceraldehyde 3-phosphate dehydrogenase), ENO (enolase 1), PKM2 (pyruvate kinase M2), and LDHA (lactate dehydrogenase A) (39) (Figure 4A). To explore whether BARX2 could affect these metabolism-related enzymes, we used qRT-PCR. The results demonstrated the mRNA and protein level of HK2 was dramatically downregulated by BARX2 siRNA transfection (Figure 4B,4C). HK2 is a cancer-related isoenzyme that catalyzes the first rate-limiting step of glucose metabolism and is a crucial key link in integrating energy production, protecting mitochondrial integrity and cell survival (40). To confirm whether BARX2 functions via expression of HK2, the plasmid overexpress HK2 was transfected into PC9 cells and treated with si-BARX2 for rescue experiments. The results showed that overexpression of HK2 could reverse the ability of cell proliferation, migration, and invasion compared to the si-BARX2 group (Figure 4D-4F). Similar findings were noted for the metabolic phenotype, including glucose consumption, lactate, pyruvate, and ECAR production (Figure 4G-4J). These results indicated that BARX2 promoted tumor progression and glucose metabolism reprogramming of LUAD cells via upregulating HK2.
HK2 is a transcriptional target of BARX2 in LUAD
Transcription factors generally induce or repress gene expression through binding to their promoters. Therefore, we hypothesized that HK2 is a transcriptional target of BARX2. As predicted by the Jaspar Database (41), we found a series of putative BARX2 binding sites in the HK2 promoter with a calculated score of 10.759–5.83968 (Figure S3A). To confirm the hypothesis, we performed ChIP assay in LUAD cells to determine whether BARX2 could bind to the HK2 promoter. A series of three sets of primers were designed to correlate with certain sites in the HK2 promoter region (Figure 5A). The results showed that a component of the -1693 bp -1704 bp (Site1) site of the HK2 promoter was enriched in chromosomal DNA precipitated by BARX2 in PC9 and A549 cells (Figure 5B,5C). To provide more related data, luciferase reporter assays were performed which showed the HK2 promoter region was amplified and cloned to the pGL4-basic vector and the empty pGL4-basic vector as a negative control, respectively. A mutant HK2-Luc reporter was also generated (Figure 5D). These data were in agreement with the result of ChIP experiments, indicating BARX2 markedly promotes the luciferase activity of HK2 in both PC9 and A549 cells (Figure 5E,5F). These results implied that BARX2 was bound to HK2.
BARX2 is directly regulated by the transcription factor FOXA1
We sought to identify upstream transcriptional regulators that may lead to BARX2 upregulation in LUAD using the UCSC Genome Browser and Jaspar Database. We predicted a series of transcription factors that bind to the BARX2 promoter region. After screening, the transcription factor FOXA1 attracted our interest as it can bind and open chromatin and trigger target transcription of target genes (42) and is also frequently amplified or mutated in human cancers (43,44). We verified that silencing FOXA1 could down-regulate the expression of BARX2 in PC9 cells (Figure 6A,6B). To understand whether BARX2 was a direct target of FOXA1, we analyzed the BARX2 promoter sequence using the JASPAR CORE database (Figure S3B). A series of three sets of primers were designed to correlate with certain sites in the BARX2 promoter region (Figure 6C). ChIP assay revealed that FOXA1 is mainly bound to the positions −515 to −529 bp (Site2) of the BARX2 promoter (Figure 6D). Moreover, the result of the luciferase reporter was consistent with the ChIP assay and showed FOXA1 could markedly promote the luciferase activity of BARX2 markedly in PC9 cells (Figure 6E,6F). Taken together, our data revealed transcription factor FOXA1 as a critical regulator up-regulating BARX2 at the transcriptional level in PC9 cells by directly binding to the BARX2 promoter.
Discussion
Lung cancer is still a major health problem due to its high incidence and mortality rates. NSCLC accounts for 85% of all lung cancer (2). Even though extensive lung cancer studies have been conducted, the underlying pivotal molecular mechanism remains to be fully elucidated, hampering therapeutic strategies. As well known, solid tumors require high energy and nutrient supplies to support their rapid growth and reproduction processes. Moreover, glucose is the primary carbon source for cancer cells to fuel energy production and biosynthetic reactions (45). To survive, tumor cells must adapt to the harsh environments of nutrient stress and reduced pH and oxygen by increasing glucose uptake and lactate production; known as the Warburg effect (4). The increase in lactate production strongly supports diverse cancer cell activities, including cell proliferation, angiogenesis, and tumor invasion activity, promoting tumor aggressiveness (46,47). HIF-1 is a crucial element in the Warburg effect, which promotes disconnection of the TCA cycle from glycolysis and reductive glycolysis. A decrease in mitochondrial activity and an increase in membrane exchangers maintain an alkaline pH in the cell, which is favorable to glycolysis and cell cycle progression (48,49). Cancer stem cells that are inherently drug-resistant are often found in hypoxic niches and depend on glycolysis for survival (50). Although metabolic reprogramming has long been observed as a feature of neoplasia and tumor growth, the mechanism driving this process remains unclear. In this study, we attempted to target tumor therapy against LUAD from the point of glucose metabolism.
The transcription factor BARX2 is a critical member of the Bar family of proteins and is expressed in some cancers. However, data regarding the role of BARX2 in tumorigenesis has been somewhat conflicting (51,52). Interestingly, Chen et al. (53) showed the BARX2 expression level was down-regulated in NSCLC. Therefore, BARX2 has attracted our interest for further research.
In contrast to the study by Chen et al. (53) our results showed that BARX2 was frequently up-regulated in LUAD tissues compared to non-neoplastic tissues. Furthermore, the TCGA database demonstrated that BARX2 may be a potential predictive marker for LUAD tissues. In addition, clinical analysis revealed that upregulation of BARX2 correlated with advanced tumor stage and poor prognosis of LUAD patients. Moreover, knockdown of BARX2 significantly inhibited proliferation, invasion, migration, and glucose metabolism ability of LUAD cells in vitro and in vivo. These data imply that BARX2 plays a vital role in promoting cancer progression, metastasis, and glucose metabolism of LUAD.
The GSEA results showed BARX2 was related to glycose metabolism-related pathways. Tumor cells reprogram energy metabolism to facilitate cell growth and proliferation, a phenomenon which has been firmly established as a classical hallmark of cancer (4). The oncogenic metabolic reprogramming is driven by essential key enzymes including HK2, GPI, PFKL, ALDO, GAPDH, ENO, PKM2, and LDHA (54). Therefore, qRT-PCR and Western blotting were performed to validate the series of glycolytic enzymes. HK2, one protein remarkably decreased by BARX2 knockdown, attracted our interest. HK2 is a crucial enzyme that catalyzes the rate-limiting first step of glycolysis and is highly up-regulated in multiple human tumors (40). In addition, mechanistic investigations found that the expression of BARX2 transcriptionally regulated HK2. We speculated that BARX2 bound to the HK2 promoter region promotes LUAD growth and metastasis.
While our study generated important and interesting findings, our results should be interpreted with caution. First, we found that the expression of BARX2 was up-regulated in LUAD from TCGA database, then verified the up-regulation of BARX2 expression in LUAD samples, LUAD tissue microarray, and LUAD cells, and verified that BARX2 plays a carcinogenic role in LUAD through experiments in vitro and vivo. However, the results by Chen et al. (53) were different from ours. We speculated that there are several reasons: the LUAD samples we used come from different institutions and may be different; in addition, the cell lines we selected were different.
In summary, our findings first defined BARX2 as a tumor promoter in LUAD and identified the BARX2/FOXA1/HK2 axis as a pivotal pathway involved in its development and progression of LUAD (Figure 7). Targeting this axis could be a novel therapeutic strategy against LUAD.
Acknowledgments
The authors thank the Jiangsu Institute of Cancer Research and Jiangsu Key Laboratory of Molecular and Translational Cancer Research for providing technical platform support. The authors appreciate the academic support from the AME Thoracic Surgery Collaborative Group.
Funding: This research was supported by the National Natural Science Foundation of China (No. 82172642), National Natural Science Foundation for Youth of China (No. 81902354), National Natural Science Foundation for Youth of China (No. 81702444), and the Excellent Talents Fund Project of Xuzhou Medical University (No. XYFY2020017).
Footnote
Reporting Checklist: The authors have completed the ARRIVE reporting checklist. Available at https://tlcr.amegroups.com/article/view/10.21037/tlcr-22-465/rc
Data Sharing Statement: Available at https://tlcr.amegroups.com/article/view/10.21037/tlcr-22-465/dss
Conflicts of Interest: All authors have completed the ICMJE uniform disclosure form (available at https://tlcr.amegroups.com/article/view/10.21037/tlcr-22-465/coif). Prof. RAdM received research grant from CNPQ Brazil, Libss, Pfizer; royalties from Springer; consulting fee from Takeda; speaker fee from Merck, Pfizer, Novartis, Eurofarma, MSD, Bayer, Astrazenenca; supporting for attending meetings and stock from Merck. Prof. RAdM also serves in Advisory board for European School of Oncology and Brazilian Society of Cancerology. ACR received Royalties from educational material for thymic tumors; payment from Honorarium for grand rounds at NY Langone; and Travel support for visiting professorship at NYU Langone; none of them are pertinent to this publication. The other authors have no conflicts of interest to declare.
Ethical Statement: The authors are accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved. The study was conducted in accordance with the Declaration of Helsinki (as revised in 2013). The study was approved by ethics board of Jiangsu Cancer Hospital. Informed consent was taken from all the patients. Animal experiments were approved by the ethics board of Nanjing Medical University (No. 2018-122), in compliance with Chinese guidelines for the care and use of animals.
Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0/.
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